Inhibitory Effect of Topical Cartilage Acellular Matrix Suspension Treatment on Neovascularization in a Rabbit Corneal Model

摘要

Physical characterization of the NCT and dg-CAM powders. A Microscopic morphology of the ECM powders. Scale bars indicate 50 μm at × 1000 magnification in the SEM images (upper panel) and at × 400 magnification in the bright field images (bottom panel). B Size distribution of each ECM particle. C Zeta potential of the ECM powders dispersed in PBS at 1 mg/ml. D Water contact angles of the ECM powders in a disk-shape was measured to examine their hydrophilicity. E Amount of soluble proteins in the ECM powders. F Gross image of the ECM powders in suspension. Images were taken at 10 h after suspending each ECM in PBS at 1 mg/ml. G The dispersion stability of the ECM powders was determined by measuring the turbidity of each ECM suspension in PBS at 1 mg/ml. H Complex viscosity of the ECM powders suspended in PBS at increasing concentrations as indicated. All data are presented as the mean ± SD from 3 independent experiments (n = 3). For statistical comparison, mean values of the particle size, zeta potential, contact angle and soluble protein contents were analyzed with unpaired two-tailed t-test and those of the viscosity test was by one-way ANOVA with Turkey’s multiple comparisons test (**p < 0.01 and ***p < 0.001)