PERK mediates resistance to BRAF inhibition in melanoma with impaired PTEN

摘要

a The western blotting showed the protein level of PTEN, pAKT, and AKT in BRAFV600E human melanoma cell lines with various PTEN genetic backgrounds. The protein level of β-actin is the loading control. b The values of 50%-inhibitory concentration (IC50) of 4 pairs of parental and resistant human melanoma cells were determined using CCK8 assays. Error bars denote s.d. for biological three repeats. Results are statistically significant between parental and resistant groups by Student’s t-test (*p < 0.05, **p < 0.01, ***p < 0.001). c The cell viability curves of 4 pairs of parental (WM88, A375sm, Sk-mel28, and WLH6215) and resistant (WM88R, A375smR, Sk-mel28R, and WLH6215R) human melanoma cells were determined using CCK8 assays. Error bars denote s.d. for biological three repeats. d The western blotting showed the indicated protein level in BRAF inhibitor-resistant melanoma cell lines with various PTEN genetic backgrounds, the protein level of tubulin as the loading control.