Single-cell and spatial analyses of cancer cells: toward elucidating the molecular mechanisms of clonal evolution and drug resistance acquisition

摘要

Scheme of the study using scRNA-seq, CyTOF, and IHC. To demonstrate the potential of the integrated analysis, we analyzed PBMCs and endoscopically and surgically resected samples from patients with gastrointestinal cancer (a). We used microdroplet-based single-cell system, 10x Genomics Chromium, for scRNA-seq analysis (b). We focused on regulatory T cell and revealed subgroup of them (c). In addition, we conducted mass cytometry using CyTOF Fluidigm (d)