Environmental conditions dictate differential evolution of vancomycin resistance in Staphylococcus aureus

摘要

a Experimental design. Wild-type (WT) S. aureus TCH1516 was used as starting strain along with strains which were initially adapted to each media condition12. Strains were tolerized under CA-MHB (blue) and RPMI+ (yellow) media conditions with increasing concentrations of vancomycin (red). Isolate naming from each lineage is listed. b Plots showing the stepwise increase of vancomycin throughout the TALE experiments. * denotes hyper-mutators. STM: Staphylococcus aureus adapted to CA-MHB. STR: Staphylococcus aureus adapted to RPMI+. SVAM: Staphylococcus aureus tolerized to vancomycin in CA-MHB. SVAR: Staphylococcus aureus tolerized to vancomycin in RPMI+. CCDs: cumulative cell divisions.