GSDME enhances Cisplatin sensitivity to regress non-small cell lung carcinoma by mediating pyroptosis to trigger antitumor immunocyte infiltration

摘要

The immune-regulation role of GSDME enhances CDDP sensitivity to regress NSCLC. a Immunohistochemical (IHC) staining of GSDME in a lung cancer tissue microarray (TMA) that contained 90 specimens, GSDME expression was analyzed in paired NSCLC lung cancer and its surrounding tissue, representative images of IHC staining were shown. b The difference of survival time and death number in NSCLC patients with low- and high-GSDME tumors that accepted chemotherapy with platinum. c Representative scanning electronic micrographs of H1299 cells with negative control or GSDME-overexpression lentivirus and then treated with cisplatin. d Representative scanning electronic micrographs of A549 cells with scrambled shRNA or GSDME shRNA lentivirus and then treated with cisplatin. e The representative image and statistical data in nude mice bearing LLC xenografts contained with negative control or GSDME-overexpressing lentivirus. f The representative image and statistical data in C57 mice bearing LLC xenografts contained with negative control or GSDME-overexpressing lentivirus. g CD3+, CD49b+, CD11c+, and F4/80+ cell number were analyzed by flow cytometry in mice with indicated LLC tumors treated with cisplatin. h Heatmap of cytokines in mice blood with indicated LLC tumors. i The statistical data of MIP-1α, MIP-1β, MIP-2, and IP-10 levels in mice blood. j A schematic representation of proposed mechanism for the role of GSDME in NSCLC