Comparative analysis of human microglial models for studies of HIV replication and pathogenesis

摘要

Schematic diagram illustrating the process and morphology of four different microglial cell types. Top panel: iPSCs progenitors undergo hematopoiesis (days 0–12) to form CD43+HPCs, then differentiate to form iPSC-MG Precursors (days 12–37), before finally maturing to form terminally differentiated iPSC-MG on day 40. Phase contrast images are shown below. Scale bar = 30 µm. Middle panel: Schematic showing differentiation of monocytes to MMG using a cytokine cocktail comprising IL-34, GM-CSF, M-CSF, Beta-NGF and CCL2. Scale bar = 18 µm. Bottom panel: Culture conditions for C20 and HMC3 cell types along with phase contrast images for these two cell types, scale bar = 25 µm