Distinct promoter methylation patterns of LKB1 in the hamartomatous polyps of Peutz-Jeghers syndrome and its potential in gastrointestinal malignancy prediction

摘要

Bisulfite PCR-Sanger sequencing revealed elevated methylation level in the hamartomatous polyps of PJS patients compared with normal mucosa. a, b Histology of PJS polyp samples used in this study, magnification = 100x, n = 50 c, d Histology of normal colon mucosa used in this study, magnification = 100x, n = 50 e Bisulfite PCR Primer design from LKB1 promoter by MethPrimer. f Representative of gel image after bisufite PCR amplifications. The PCR product is 259 bp, n = 100 (g) Average methylation level for LKB1 promoter region, comparison between 50 PJS polyps and 50 normal mucosa samples revealed the gap between two groups. Means ± SEM, *P < 0.05. h The methylation analysis per each CpG site indicated that instead of randomly distributed, DNA methylation was evenly distributed across the whole region. Data presented as means. All bars = 100 μm