A nanoparticle vaccine that targets neoantigen peptides to lymphoid tissues elicits robust antitumor T cell responses

摘要

a C57Bl/6 mice were immunized with indicated peptides on days 0 and 7. Reactivity was determined by ELISpot using splenocytes re-stimulated on day 14. b Mice immunized as in a with G12C1–23, G12D1–23, or G12V1–23 and re-stimulated with the immunizing peptide or 9 or 15-mer. c Splenocytes from G12D1–23-immunized mice were re-stimulated with WT or G12C 23-mer peptides. d IFN-γ ELISpot results after co-culture of BMDCs pulsed with the immunizing peptide with CD8+ T cells from the spleens of G12C1–23-, G12D1–23-, and G12V1–23-immunized mice. e Peptide-specific IFN-γ secretion from CD4+ T cells co-cultured with peptide-pulsed BMDCs. f, g G12D1–23 peptide-specific CD4+ T cells measured by intracellular cytokine staining by cytometry. Significance determined using two-way ANOVA (a, c) and paired two-tailed Student’s t-test (***p < 0.001, **p < 0.01, *p < 0.05. Error bar = mean ± s.e.m.) (a-e, g). Results from at least two independent studies with two to five mice each.