Heterologous redox partners supporting the efficient catalysis of epothilone B biosynthesis by EpoK in Schlegelella brevitalea

摘要

Highly efficient redox proteins support the catalytic activity of EpoK in DSM 7029. a Epothilone yield of strain H7029-2 with epoK expression. The data are presented as the means ± standard deviations of the values from three independent experiments (n = 3). b HPLC analysis of the standard sample (25 mg L−1) and the extracts from the parental strain H7029-1 and the mutant strain H7029-2. A, epothilone A; B, epothilone B; C, epothilone C; D, epothilone D. c Schematic diagram: EpoK efficiently catalysed the conversion of epothilone C and D to epothilone A and B, respectively, in the epothilone C/D high-yield strain H7029-1. The epothilone gene cluster from S. cellulosum So0157-2 was transposed into the genome of the heterologous host DSM 7029