Compared with previous Miniscope and MiniLFM designs, our Miniscope3D is lighter weight and more compact. We remove the Miniscope’s tube lens and place a 55 μm thick optimized phase mask at the aperture stop (Fourier plane) of the GRIN objective lens. A sparse set (64 per depth) of calibration point spread functions (PSFs) is captured by scanning a 2.5 μm green fluorescent bead throughout the volume. We use this data set to pre-compute an efficient forward model that accurately captures field-varying aberrations. The forward model is then used to iteratively solve an inverse problem to reconstruct 3D volumes from single-shot 2D measurements. The 3D reconstruction here is of a freely swimming fluorescently tagged tardigrade
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