406 Reduce Reuse Rerumen: Reduction of Eschericia coli coliform bacteria Enterobacteriaceae and yeast and mold colonies in dried paunch manure

摘要

The discovery of novel by-product feeds for animals, especially cattle, supports an economically viable agricultural community and enhanced stability in the United States food chain. By-products of livestock processing (in this case, paunch manure, or rumen content) could provide the tools necessary to achieve this goal. Paunch manure, the material from ruminant stomachs at the time of harvest, is a waste product of the meat industry and represents a final loss due to cost of disposal. Our objective in this study was to determine the microbial content of fresh versus dried paunch manure in an effort to assess viability as a potential feed source. Fresh paunch manure was collected from cattle at a local abattoir and immediately homogenized for microbial plating. One milliliter of decanted paunch manure was plated on specialized film for determination of colony counts from Enterobacteriaceae, coliform-forming bacteria, Eschericia coli, Salmonella, and yeast and mold. Plates were incubated at 36°C for 24 ± 2 hours. Data were analyzed as a random effects model using PROC MIXED of SAS v. 9.4. In the wet paunch, E. coli had a mean colony count of 3, coliform bacteria of 53, yeast and mold of 0, and Enterobacteriaceae were too numerous to count. In the dry paunch, E. coli had a mean colony count of less than 1, coliform bacteria of 52, yeast and mold of 0, and Enterobacteriaceae less than 1. Source of paunch contributed a majority to the total variance in all instances of the random effects models. Results are interpreted to mean that, given the drastic reduction in microbial loads, paunch manure may represent a viable feedstock for further testing and development.