Enhancement of sialylation in rIgG in glyco-engineered Chinese hamster ovary cells

摘要

a The expression levels of the investigated genes were evaluated using quantitative RT-PCR in both wild-type and stable transfected cell lines. Left column: ST6GAL-I; right column: GNE; P < 0.05. b Gel electrophoresis of the investigated genes’ PCR products using specific primers in the WT, transfected CHO cells. Control: plasmid pcDNA3.1-SG2 (Thi Sam et al. 2018)