The present invention relates to a method for purifying spI glycoprotein of chickenpox virus (VZV) expressed from recombinant CHO cells, wherein the culture of recombinant CHO cells, centrifugation of the medium, dialysis of the supernatant, and anion exchange chromatography of the dialysate are performed. According to the method including the step, a large amount of gpI glycoprotein of high purity VZV can be obtained.
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