MYB repressors and MBW activation complex collaborate to fine-tune flower coloration in Freesia hybrida

摘要

a Multiple alignment of the amino acid sequences of anthocyanin or proanthocyanidin related MYB repressors. Modified version of FhMYB27 was included: FhMYB27ΔC (C-terminal repression domain removed). Conserved residues were indicated by asterisk and partial conservations were indicated by: or ·. The numbers indicated the positions of the last residue in each line. The R2 and R3 domains were indicated by overlines. The shaded residues in R2 and R3 domains indicated the three helixes with different colors, respectively. The conserved [D/E]Lx2[R/K]x3Lx6Lx3R motif responsible for interacting with bHLH factors were boxed with rectangular frames. The conserved C1, C2, C3, C4, and C5 motifs in the C-terminus of different MYB repressors were outlined with different colors. Red stars indicated the Freesia MYB regulators isolated in this study. b Phylogenetic analysis of amino acid sequences of MYB proteins in Freesia and other species. The Freesia FhMYB27 and FhMYBx were highlighted with the red stars. The tree was constructed using Maximum Likelihood method and Poisson correction model by MEGA version X. The human c-MYB sequence ({"type":"entrez-protein","attrs":{"text":"NP_001155129","term_id":"239735490","term_text":"NP_001155129"}}NP_001155129) was used for the outgroup, and a selection of non-flavonoid-related R2R3MYBs from Marchantia polymorpha (MpMYB09 {"type":"entrez-protein","attrs":{"text":"PTQ41991.1","term_id":"1376851403","term_text":"PTQ41991.1"}}PTQ41991.1; MpMYB13 {"type":"entrez-protein","attrs":{"text":"PTQ35332.1","term_id":"1376844704","term_text":"PTQ35332.1"}}PTQ35332.1; MpMYB17 {"type":"entrez-protein","attrs":{"text":"PTQ32714.1","term_id":"1376842062","term_text":"PTQ32714.1"}}PTQ32714.1) were included for comparison. Nodes with bootstrap no <40% from 1000 replicates were shown. The following GenBank accession numbers were used: Antirrhinum majus AmMYB308 ({"type":"entrez-protein","attrs":{"text":"P81393","term_id":"75107028","term_text":"P81393"}}P81393), AmMYB330 ({"type":"entrez-protein","attrs":{"text":"P81395","term_id":"75107030","term_text":"P81395"}}P81395); Arabidopsis thaliana AtPAP1 ({"type":"entrez-protein","attrs":{"text":"AAG42001","term_id":"11935171","term_text":"AAG42001"}}AAG42001), AtPAP2 ({"type":"entrez-protein","attrs":{"text":"AAG42002","term_id":"11935173","term_text":"AAG42002"}}AAG42002), AtTT2 ({"type":"entrez-protein","attrs":{"text":"Q2FJA2","term_id":"123487077","term_text":"Q2FJA2"}}Q2FJA2), AtMYB11 ({"type":"entrez-protein","attrs":{"text":"NP_191820","term_id":"15228811","term_text":"NP_191820"}}NP_191820), AtMYB12 ({"type":"entrez-protein","attrs":{"text":"CAB09172","term_id":"2832377","term_text":"CAB09172"}}CAB09172), AtMYB111 ({"type":"entrez-protein","attrs":{"text":"NP_199744","term_id":"15239855","term_text":"NP_199744"}}NP_199744), AtMYB4 ({"type":"entrez-protein","attrs":{"text":"AAC83582","term_id":"3941412","term_text":"AAC83582"}}AAC83582), AtMYB7 ({"type":"entrez-protein","attrs":{"text":"NP_179263","term_id":"15227288","term_text":"NP_179263"}}NP_179263), AtMYB32 ({"type":"entrez-protein","attrs":{"text":"NP_195225","term_id":"15236236","term_text":"NP_195225"}}NP_195225), AtMYB5 ({"type":"entrez-protein","attrs":{"text":"AAC49311","term_id":"1218000","term_text":"AAC49311"}}AAC49311), AtCPC ({"type":"entrez-protein","attrs":{"text":"NP_182164","term_id":"15225977","term_text":"NP_182164"}}NP_182164); Vitis vinifera VvMYBF1 ({"type":"entrez-protein","attrs":{"text":"ACT88298","term_id":"254940122","term_text":"ACT88298"}}ACT88298), VvMYB5a ({"type":"entrez-protein","attrs":{"text":"AAS68190","term_id":"45593281","term_text":"AAS68190"}}AAS68190), VvMYB5b ({"type":"entrez-protein","attrs":{"text":"Q58QD0","term_id":"75320448","term_text":"Q58QD0"}}Q58QD0), VvMYBPA1 ({"type":"entrez-protein","attrs":{"text":"CAJ90831","term_id":"130369073","term_text":"CAJ90831"}}CAJ90831), VvMYBPA2 ({"type":"entrez-protein","attrs":{"text":"ACK56131","term_id":"217795196","term_text":"ACK56131"}}ACK56131), VvMYB4 ({"type":"entrez-protein","attrs":{"text":"NP_001268129","term_id":"526117848","term_text":"NP_001268129"}}NP_001268129); Petunia hybrida PhMYB4 ({"type":"entrez-protein","attrs":{"text":"ADX33331","term_id":"323149963","term_text":"ADX33331"}}ADX33331), PhMYB27 ({"type":"entrez-protein","attrs":{"text":"AHX24372","term_id":"613399455","term_text":"AHX24372"}}AHX24372), PhMYBx ({"type":"entrez-protein","attrs":{"text":"AHX24371","term_id":"613399453","term_text":"AHX24371"}}AHX24371); Lilium hybrid LhMYB6 ({"type":"entrez-protein","attrs":{"text":"BAJ05399","term_id":"294679643","term_text":"BAJ05399"}}BAJ05399); Zea mays MYB31 ({"type":"entrez-protein","attrs":{"text":"NP_001105949","term_id":"806638669","term_text":"NP_001105949"}}NP_001105949), ZmMYB42 ({"type":"entrez-protein","attrs":{"text":"NP_001106009","term_id":"894216153","term_text":"NP_001106009"}}NP_001106009); Diospyros kaki DkMYB4 ({"type":"entrez-protein","attrs":{"text":"BAI49721","term_id":"269784590","term_text":"BAI49721"}}BAI49721); Fragaria ananassa FaMYB1 ({"type":"entrez-protein","attrs":{"text":"AAK84064","term_id":"15082210","term_text":"AAK84064"}}AAK84064); Populus tremula x Populus tremuloides PtrMYB182 ({"type":"entrez-protein","attrs":{"text":"AJI76863","term_id":"754295703","term_text":"AJI76863"}}AJI76863), PtrMYB134 ({"type":"entrez-protein","attrs":{"text":"ACR83705","term_id":"239616062","term_text":"ACR83705"}}ACR83705); Eucalyptus gunnii EgMYB1 ({"type":"entrez-protein","attrs":{"text":"CAE09058","term_id":"39725415","term_text":"CAE09058"}}CAE09058); Panicum virgatum PvMYB4a ({"type":"entrez-protein","attrs":{"text":"AEM17348","term_id":"343381807","term_text":"AEM17348"}}AEM17348); and Vaccinium uliginosum VuMYBR3 ({"type":"entrez-protein","attrs":{"text":"AKR80572","term_id":"902573607","term_text":"AKR80572"}}AKR80572).