MiR-200b in heme oxygenase-1-modified bone marrow mesenchymal stem cell-derived exosomes alleviates inflammatory injury of intestinal epithelial cells by targeting high mobility group box 3

摘要

a–c HO-1/BMMSCs and GFP/BMMSCs were established by transfection of Adenovirus-Ho-1 and Adenovirus-Gfp. The relative expression levels of the mRNA and protein of HO-1 in HO-1/BMMSCs were verified using qRT-PCR (a, fold change relative to BMMSCs, n = 4). Western blotting (b, n = 3) and immunofluorescence (c, n = 3). d The number of DEGs between HO-1/BMMSCs and GFP/BMMSCs. e A heatmap was used to represent the DEGs in HO-1/BMMSCs compared with GFP/BMMSCs. f, g Go analysis (f) and KEGG analysis (g) of the DEGs showed the biological function and signal pathways of BMMSCs regulation after Ho-1 modification. h QRT-PCR validation of the mRNA levels of selected DEGs to verify the results of RNA-sequencing, including Ifit1, Akap2, Nlrc5, Rgs7, Cdh1, and Snip1 (fold change relative to BMMSCs, n = 3). *P < 0.05. Akap2 A-kinase anchoring protein 2; BMMSCs Bone marrow mesenchymal stem cells, Cdh1 Cadherin 1; DEGs differentially expressed genes; FDR false discovery rate; GFP green fluorescent protein; GO Gene ontology; HO-1 heme oxygenase-1; Ifit1 Interferon-induced protein with tetratricopeptide repeats 1; KEGG Kyoto Encyclopedia of Genes and Genomes; Nlrc5 NLR family, CARD domain containing 5; Snip1 Smad nuclear interacting protein 1; TPM transcripts per million reads; qRT-PCR quantitative real-time reverse transcription polymerase chain reaction.