The differential immune responses to COVID-19 in peripheral and lung revealed by single-cell RNA sequencing

摘要

a The cartoon outlines the study design. PBMC and BALF cells from COVID-19 patients and healthy controls were collected for scRNA-seq characterization using the 10× Genomics platform. The number of samples, analyzed cells, and samples with PBMC and BALF cells simultaneously collected from the same patient are indicated. HC, healthy controls; Mild, mild patients; Severe, severe patients. b The UMAP projection of the combined PBMC scRNA-seq dataset identifies nine major cell types. PC, plasma cells. c The specific markers for identifying each immune cell types in b are indicated. (Pct.Exp. indicates percentage of cells expressed). d Density plots show the UMAP projection of PBMCs from COVID-19 patients and controls. e The bar plot shows the proportions of each cell types in PBMCs from individual subjects. The cell numbers and ratios of monocyte/T cells are listed to the right side. f Comparisons of percentages of each cell types in PBMCs (cycling cells were re-clustered into T and PC subsets) between the two COVID-19 groups and controls (two-sided Student’s t-test, *P < 0.05, **P < 0.01, ***P < 0.001. H, healthy controls; M, mild patients; S, severe patients.