Functional expression of a novel methanol-stable esterase from Geobacillus subterraneus DSM13552 for biocatalytic synthesis of cinnamyl acetate in a solvent-free system

摘要

Conserved sequence alignment and structure analysis of EstGSU753. (a) Conserved sequence alignment of EstGSU753. The structures are denoted as follows: ▲, the catalytic site (Ser-97, Asp-196 and His-226). The conserved region, Gly-Xxx-Ser-Xxx-Gly was boxed. (b) The whole three-dimensional structure. The three-dimensional structure of EstBAS was predicted by the SWISS-MODEL server. (c) The catalytic site of EstBAS structure. The catalytic triad: Ser-97, Asp-196 and His-226 residues are marked in gray