LncRNA SNHG14 promotes OGD/R-induced neuron injury by inducing excessive mitophagy via miR-182-5p/BINP3 axis in HT22 mouse hippocampal neuronal cells

摘要

SNHG14 overexpression promotes apoptosis of OGD/R-induced HT22 cells. HT22 cell model was established by OGD/R treatment. Normal HT22 cells served as control. a QRT-PCR was performed to assess the expression of SNHG14 in the HT22 cells. HT22 cells were transfected with pcDNA3.1-SNHG14, pcDNA3.1-NC, si-SNHG14 or si-Ctrl. Then, the modified HT22 cells were subjected to OGD/R treatment. b QRT-PCR was performed to detect the expression of SNHG14 in the modified HT22 cells. c Flow cytometry was performed to explore the apoptosis of the modified HT22 cells. d The expression of caspase-3, cleaved-caspase-3, caspase-9 and cleaved-caspase-9 in the modified HT22 cells was examined by WB. (*P < 0.05, **P < 0.01, versus Control or Vector; #P < 0.05, ##P < 0.01, versus si-Ctrl)