Wingless and Archipelago a fly E3 ubiquitin ligase and a homolog of human tumor suppressor FBW7 show an antagonistic relationship in wing development

摘要

ago is necessary for development of chemosensory bristles at wing margin. In all figures, chemosensory bristles with shafts and without shafts are marked with black arrows and red arrowheads, respectively. a-f Phenotypes of chemosensory bristles in control ap-Gal4/+ (n = 32, a), ap > ago RNAi (agoHM04005, agoHMS00111) (n = 26, b, and n = 46, c), ap > ago (agoUAS.ORF, myc-ago) (n = 18, d, and n = 24, e) and ap > agoHMS00111myc-ago (n = 21, f) wings. Images were taken by focusing on sensory bristles at dorsal anterior wing margin. Proximal to the left. Portions of the wings in (a-f) are magnified in (a'-f') and further magnified images in insets are to show chemosensory bristles. g Numbers of mechanosensory and chemosensory bristles along the anterior wing margin from proximal to distal tip of the longitudinal vein 2 were counted, and the data are presented in the bar graph (***p < 0.001 and ****p < 0.0001). The blue and orange bars represent numbers of mechanosensory and chemosensory bristles, respectively. h Whole wing images as shown in Additional file: Fig. S2 were used to calculate wing size (****p < 0.0001)