Metabolic engineering of Aspergillus niger via ribonucleoprotein-based CRISPR–Cas9 system for succinic acid production from renewable biomass

摘要

Proposed metabolic pathway for succinic acid production by A. niger and strategy for strain engineering (the SAP-3 strain). PYC, pyruvate carboxylase; MDH, malate dehydrogenase; FUM, fumarase; FRD, fumarate reductase; AnDCT, C4-dicarboxylate transporter from A. niger; AcDCT, C4-dicarboxylate transporter from A. carbonarius; PDH, pyruvate dehydrogenase; GOX, glucose oxidase; OAH, oxaloacetate hydrolase; TCA cycle, tricarboxylic acid cycle