Enzymological characterization of a novel d-lactate dehydrogenase from Lactobacillus rossiae and its application in d-phenyllactic acid synthesis

摘要

Alignment of multiple deduced amino acid sequences of LrLDH and LDHs from other sources. The conserved sites are all labeled in red, the catalytic amino acids, including Arg241, Glu270 and His303 (LrLDH numbering), are marked with stars, and the NADH binding domain Gly158 (x) Gly160 (xx) Gly163 (17x) Asp181 (LrLDH numbering) is marked with triangle. The GenBank ID of LDHs were labeled on the left