In vitro shoot multiplication of Rumex vesicarius L. and quantification of ascorbic acid and major phenolics from its leaf derived callus

摘要

In vitro cultures of R. vesicarius.a In vitro seedlings (bar = 12 mm) obtained with 2 weeks of culturing. b Proliferation of 2–3 shoots from shoot tip explants on MS medium-containing 1 mg L−1 BA (bar = 12 mm) with 4–6 weeks of culturing. c Multiple shoot formation from shoot tip explant in presence of 2 mg L−1 kinetin (bar = 12 mm) with 3 weeks of culturing. d Shoot elongation along with simultaneous callusing on ½ MS medium with 0.5 mg GA3 (bar = 8 mm) with 3 weeks of culturing. e Lush green long shoots with broad leaves in the presence of 10 µM AgNO3, 0.5 mg L−1 IAA, and 2 mg L−1 BA (bar = 12 mm) with 3 weeks of culturing. f In vitro rooting on MS medium with 1.5 mg L−1 IBA and 1% activated charcoal (bar = 8 mm) with 3–4 weeks of culturing. g 2 month old potted tissue cultured plant (bar = 45 mm)