Development of EphA2 siRNA-loaded lipid nanoparticles and combination with a small‐molecule histone demethylase inhibitor in prostate cancer cells and tumor spheroids

摘要

Overview of steps involved in the preparation of cSLNs and their complexes with siRNA. (Steps 1 and 2) Preparation of empty cSLN formulations, which have a solid core at room temperature. (Steps 3 and 4) Addition of negatively charged siRNAs to the pre-formed cSLNs and incubation of this mix for complex formation at the positively charged outer surface of particles via electrostatic interaction