Optimal double-spin method for maximizing the concentration of platelets inequine platelet-rich plasma

摘要

This study optimized the double-spin conditions for preparing equine platelet-rich plasma(PRP): leukocyte-rich PRP (L-PRP) and leukocyte-poor PRP (P-PRP). Whole blood samples werecentrifuged at various double-spin conditions. Both L-PRP and P-PRP were prepared at eachstage, and complete blood counts and growth factor concentrations were compared. Samplescentrifuged at 160 × 900 g, 160 × 2,000 g, and 400 × 2,000 g exhibited the highestplatelet counts. P-PRP had significantly lower leukocyte and erythrocyte contents thanL-PRP, especially at 400 × 2,000 g. No significant differences were observed in growthfactor concentrations. Our data suggest that optimum L-PRP preparation should includecentrifugation under the aforementioned conditions, whereas centrifugation at 400 × 2,000g is optimal for P-PRP.