Expression of silent information regulator 1 in chronic rhinosinusitis and regulatory effects of inflammatory factors

摘要

We aimed to investigate the expression of silent information regulator 1 (Sirt1) in chronic rhinosinusitis (CRS) and the regulatory effects of inflammatory factors. The mucosal epithelial tissues of the nasal ethmoid sinus were collected from 30 patients with CRS from March 2017 to March 2019, and tissues from patients undergoing functional rhinoplasty were included as a control group. H&E staining and immunohistochemistry were performed to detect the histopathologic changes in the nasal mucosa and the expression of Sirt1. Epithelial cells in the control group were extracted from the ethmoid sinus mucosa and cultured in vitro. After the cells were treated with 0, 1, 10, and 100 ng/mL interleukin-5 (IL-5) and interferon-gamma (IFN-γ) for 24 h, qRT-PCR and western blotting were carried out to detect the mRNA and protein expressions of Sirt1. Nasal mucosal tissues of the control group were complete in structure, whereas large quantities of inflammatory cells infiltrated in nasal mucosa of the CRS group. Compared with the control group, the CRS group had significantly decreased protein and mRNA expression levels of Sirt1 (P<0.05), which significantly declined with increasing concentrations of IL-5 and IFN-γ (P<0.05). Thus, expression of Sirt1 in the nasal mucosa tissues of CRS patients is decreased, and inflammatory factors can reduce such expression in a dose-dependent manner. Sirt1 may participate in the inflammatory stress process of CRS.