Reproductive Soldier Development Is Controlled by Direct Physical Interactions with Reproductive and Soldier Termites

摘要

Generally, in termites, the soldier caste is sterile, and their reproductive organ formation is suppressed. However, in some primitive taxa, neotenic reproductives with soldier-like mandibles, called reproductive soldiers, occasionally appear in the incipient colony. Our first step in understanding the developmental mechanism of this unique caste was to establish efficient conditions to induce reproductive soldiers in Zootermopsis nevadensis. Removing both the king and soldiers from an incipient colony strongly stimulated the formation of male reproductive soldiers, which possessed soldier-like mandibles and developed testes. Similarly, high differentiation rates of male reproductive soldiers were observed after king-and-soldier separation treatment using wire mesh. However, no male reproductive soldiers were produced without direct interaction with the queen. These results suggest that reproductive soldier development might be initiated and controlled by direct physical interactions with both reproductives (the king and queen) and soldiers.