The nucleotide specificity of succinyl‐CoA synthetase of

摘要

Electrostatic interactions between charged gatekeeper residues of the enzyme and approaching substrates can effectively discriminate between similar substrates. We investigated whether the charged gatekeeper residues play any role in the substrate specificity of P. falciparum succinyl‐CoA synthetase (PfSCS). Our results demonstrated that charged gatekeeper residues in PfSCS did alter the ATP binding but could not alter its substrate specificity, pointing toward other factors affecting the substate specificity of PfSCS.