Neutron reflectometry and NMR spectroscopy of full-length Bcl-2 protein reveal its membrane localization and conformation

摘要

NR (neutron reflectometry) provides a global picture of the membrane, including locations of its molecular compounds and their volumetric contributions, as shown in this schematic diagram of the Bcl-2 protein (in tail-anchored and membrane-embedded positions). NMR provides complementary structural and dynamic information at atomic to entire protein molecule levels. The schematic Bcl-2 structure shown is based on a model of Bcl-2 (derived from PDB_ID 1YSW), including its fully flexible loop domain (FLD).