JmjC-KDMs KDM3A and KDM6B modulate radioresistance under hypoxic conditions in esophageal squamous cell carcinoma

摘要

a Cell surviving fraction in three ESCC cell lines irradiated with [0–8] Gy range concentration under normoxia, 50 µM CoCl2 and hypoxia through SHMT model analysis. Results are presented as mean±SD of at least 3 independent experiments. b DNA damage of 2 Gy irradiated ESCC cells between 0 and 24h, under normoxia, 50 µM CoCl2 and hypoxia by comet assay. The results are the mean of at least 50 comets per condition. All values of DNA fragmentation (tail moment) were normalized to control (0Gy). Further, hypoxic conditions (50µM CoCl2 or 0.5–1% O2) were compared to normoxia. c Representative pictures of nuclear γ-H2AX staining of 2 Gy irradiated ESCC cells under normoxia, 50 µM CoCl2 and hypoxia conditions. All pictures were taken from Olympus IX51 microscope at ×400 magnification (scale bar 20 μm). IF quantification was done using ImageJ software (version 1.6.1, from National Institutes of Health) and represented as a fold change between 2 Gy irradiated cells and non-irradiated control. IF, fluorescence intensity. d ESCC cell apoptosis under normoxia, 50 µM CoCl2 and hypoxia conditions with 2 Gy of IR. Results are presented as mean±SD of at least three independent experiments. e ESCC cell migration through wound-healing assay, after 24 h of 2 Gy treatment normalized 0 h. Results are the mean±SD of at least three independent experiments; Irradiated cells are compared to non-irradiated cells in each condition. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.