Increased expression and retention of the secretory chaperone proSAAS following cell stress

摘要

ER stress induced by tunicamycin increases the expression of BiP and proSAAS mRNAs. Tunicamycin (Tun) exposure increases mRNA levels of the ER stress marker BiP in AtT-20 cells (a), Neuro2A cells (b), and primary hippocampal neurons (c) but increases proSAAS mRNA levels only in Neuro2A cells. Cells were treated with increasing concentrations of tunicamycin (Tun) for 24 h, and mRNA levels were determined by quantitative real-time PCR. Each mRNA is shown relative to GAPDH and plotted as the fold increase compared with the vehicle control (DMSO). The data shown represent the averages of four biological and three technical replicates ± SD. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001; one-way ANOVA with the Tukey post hoc test