P2X7 receptor signaling promotes inflammation in renal parenchymal cells suffering from ischemia-reperfusion injury

摘要

A total of ten wild-type mice were randomly divided into the group Ischemia-Reperfusion (IR) (n = 5) and the group Sham (n = 5). A The levels of urine ATP, corrected for urine creatinine excretion. B The levels of P2X7 receptor mRNA in kidneys were measured by quantitative polymerase chain reaction. C Representative immunoblots and D aggregate densitometric quantification of whole kidney lysate for P2X7 receptor expression. E Representative immunofluorescent staining for IL-1β (red) and P2X7R (green) merged with 4’, 6-diamidino-2-phenylindole (DAPI, blue). HK2 cells were subjected to hypoxia for 24 h followed by reoxygenation for a determined time. F The time course for ATP concentrations in the supernatants. G Representative immunoblots and H aggregate densitometric quantification of cell lysate for IL-1β p17 expression. I The levels of P2X7 receptor mRNA in HK2 cells. J Representative immunoblots and K aggregate densitometric quantification of HK2 cell lysate for P2X7 receptor expression. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.