LncRNA DANCR represses Doxorubicin-induced apoptosis through stabilizing MALAT1 expression in colorectal cancer cells

摘要

A Changes in lncRNA expression in HCT116 and RKO cells upon Dox treatment were presented in Scatter Dot Plots. X axis: log2(FPKM) of control cells; Y axis: log2(FPKM) of Dox-treated cells. Arrows indicated lncRNAs DANCR, PURPL, and H19, whose fold changes were higher than 1.5-fold. B The expression levels of DANCR, TP53, H19, and PURPL were detected by qPCR in HCT116 (top) and RKO (bottom) cells treated with increasing dosages of Dox (0, 50, 100, 200 nM) for 24 h. *p < 0.05. C DANCR knockdown inhibited HCT116 and SW620 cells proliferation (top), whereas DANCR overexpression promoted the growth of HCT116 and SW620 cells detected by CCK8 assays (bottom). *p < 0.05. D Cell cycle progression was analyzed using flow cytometry in HCT116 and SW620 cells with shDANCR or DANCR overexpression vectors. *p < 0.05. E The anchorage-independent growth capacity of HCT116 and SW620 cells was impaired upon DANCR knockdown, and it was enhanced in HCT116 and SW620 cells overexpressing DANCR. *p < 0.05. F Relative expression levels of DANCR in colorectal cancer tissues of different pathological stages. Normal, adjacent noncancerous tissues (n = 18). I–IV: TNM stages I (n = 21), II (n = 40), III (n = 44), and IV (n = 21). *p < 0.05.