Phosphorylation by Aurora B kinase regulates caspase-2 activity and function

摘要

a Schematic diagram of phosphorylation site analysis of caspase-2. b Caspase-2 phosphorylation sites identified by LC–MS/MS. The phosphorylated amino acid position, charge, mass error, peptide location in caspase-2, phosphorylated S/T site position in caspase-2 and localisation probability are indicated. Phosphorylated residues are indicated in brackets. Spectra for the peptides in (b) can be found in Supplementary Fig. S2. c Phosphorylation sites in caspase-2 identified by mass spectrometry. Functionally reported sites are indicated in red. S (Ser) or T (Thr) (in black) indicates positions of novel potential phosphorylation sites. NLS nuclear localisation signal. C320 catalytic Cys. d Multiple amino acid sequence alignment showing conservation of phosphorylation sites in caspase-2 from different species. Newly identified and known phosphorylation sites with high homology are shaded in purple and grey, respectively.