Comparison of the effects of introducing the CRISPR/Cas9 system by microinjection and electroporation into porcine embryos at different stages

摘要

Introduction of the CRISPR/Cas9 system targeting the B4GALNT gene into embryos by electroporation. a Cleavage and b blastocyst formation rates of electroporated embryos. Five replicates were analyzed for each treatment group. c Genotype of blastocysts, determined using Sanger sequencing and TIDE analysis. The proportions were calculated by dividing the number of gene-edited (bi-allelic and mosaic) blastocysts by the total number of sequenced blastocysts. bi: blastocysts having bi-allelic mutations, mos: blastocysts having mosaic mutations. d Mutation efficiency of gene-edited blastocysts. The mean proportions represent the proportion of indel mutation events in gene-edited blastocysts. The numbers within parentheses under the X-axis indicate the total number of examined samples. Error bars indicate mean ± SEM (a, c). a−c, A−CBars with different letters differ significantly (P < 0.05)