Review on the Computational Genome Annotation of Sequences Obtained by Next-Generation Sequencing

摘要

Due to the development of high-throughput sequencing technologies, computational genome annotation of sequences has become one of the principal research area in computational biology. First, we reviewed comparative annotation tools and pipelines for both annotations of structures and functions, which enable us to comprehend gene functions and their genome evolution. Second, we compared genome annotation tools that utilize homology-based and ab initio methods depending on the similarity of sequences or the lack of evidences. Third, we explored visualization tools that aid the annotation process and stressed the need for the quality control of annotations and re-annotations, because misannotations may happen due to experimental errors or missed genes by preceding technologies. Finally, we highlighted how emerging technologies can be used in future annotations.