Ischemia induces autophagy of endothelial cells and stimulates angiogenic effects in a hindlimb ischemia mouse model

摘要

Representative pictures of the foot were taken at days 3 and 7 after HLI injury. The ischemic hindlimb was evaluated using ischemia scoring as described. (grade 0, no damage; grade 1, damaged claws; grade 2, damaged toe; grade 3, damaged to all toes; and grade 4, damaged foot). The blood flow index was examined at the indicated times using ICG. The blood flow index represents the overall blood volume information with respect to time. H&E staining was performed using cross-sectioned gastrocnemius muscle 7 days after injury. The red-dotted circle shows the necrotized region. Scale bar = 50 μm. Gastrocnemius tissue was also stained with Masson, and the red-dotted circle shows fibrosis. Scale bar = 25 μm.