. (A) Schematic representation of polyprotein pp62 catalyzed into mature structural proteins: p8, p35, and p15. The precursor pp62 and intermediate precursor pp46 are coloured in grey; the mature proteins p15, p35, and p8 are coloured in green, blue, and purple, respectively. The cleavage sites “GGG” and “GGN” are labelled on the top. (B) Purification of ASFV p35. The protein is purified over a superdex75 16/60 size-exclusion column. The black line indicates the absorbance at UV280 nm. Three peaks are corresponding to monomer, dimer and higher oligomer. (C) The calculated molecular weights corresponding to each peak in AUC are labelled above the curve. Sed stands for sedimentation. (D) SDS-PAGE gel of ASFV p35 from the fraction in the monomer peak (left), and the native gel in different solution pH (right). (E) The crystal structure of ASFV p35 is shown in cartoon. The secondary structure elements are numbered consecutively with the N terminus and C terminus labelled. The size of the ASFV p35 protein is labelled. (F) Structures of ASFV p35 protein and IASV Matrix are shown in cartoon. The topology of two structure are also presented; secondary structure elements are numbered consecutively
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