Transcriptome Comparison of Defense Responses in the Rice Variety ‘Jao Hom Nin’ Regarding Two Blast Resistant Genes Pish and Pik

摘要

Jao Hom Nin (JHN) is a Thai rice variety with broad-spectrum resistant against rice blast fungus. JHN contains two rice blast resistant genes, and , located on chromosome 1 and on chromosome 11, respectively. To understand the blast resistance in JHN, the study of the defense mechanism related to the and genes is crucial. This study aimed to dissect defense response genes between the and genes using the RNA-seq technique. Differentially expressed genes (DEGs) of and backcross inbred lines were identified between 0 and 24 h after inoculation with rice blast spore suspension. The results showed that 1248 and 858 DEGs were unique to the and lines, respectively. The wall-associated kinase gene was unique to the line and the zinc-finger-containing protein gene was unique to the line. Pathogenicity-related proteins PR-4 and PR-10 were commonly found in both and lines. Moreover, DEGs functionally categorized in brassinosteriod, jasmonic acid, and salicylic acid pathways were detected in both and lines. These unique and shared genes in the and rice blast defense responses will help to dissect the mechanisms of plant defense and facilitate rice blast breeding programs.