RIPK3 blockade attenuates tubulointerstitial fibrosis in a mouse model of diabetic nephropathy

摘要

Increased expression of receptor-interacting protein kinase (RIPK) 3 and pyrin domain-containing protein (NLRP) 3 in diabetic kidney disease. ( ) was reused from the thesis . ( ) RIPK3 mRNA expression fold change was measured by RT-PCR in mice (wild-type) +/− diabetes mellitus. Tubulin was used as the reference gene. ( ) Immunoblot analysed of kidneys from the same groups as in ( ) using antibodies for phospho-RIPK3 and Tubulin. ( ) Representative immunohistochemical staining images and the quantitation of NLRP3 expression in the renal cortex from the same groups as in ( ). Magnification: 20×. Statistical analysis was performed using two-tailed -tests. For all graphs, each dot or triangle represents an individual sample, and horizontal bars denote the mean ± S.E.M. *P