Development of reverse transcription loop-mediated isothermal amplification for rapid detection of porcine epidemic diarrhea virus

摘要

In this study, a reverse transcription loop-mediated isothermal amplification (RT-LAMP) was developed for detection of (PEDV). Six primers were designed to amplify the nucleocapsid (N) gene of PEDV. The optimization, sensitivity, and specificity of the RT-LAMP were investigated. The results showed that the optimal reaction condition for RT-LAMP amplifying PEDV N gene was achieved at 63°C for 50 min. The RT-LAMP assay was more sensitive than gel-based RT-PCR and enzyme-linked immunosorbent assay. It was capable of detecting PEDV from clinical samples and differentiating PEDV from , , , and