Detection of feline coronavirus using microcantileversensors

摘要

This work demonstrated the feasibility of detectingsevere acute respiratory syndrome associated coronavirus (SARS-CoV) usingmicrocantilever technology by showing that the feline coronavirus (FIP) type Ivirus can be detected by a microcantilever modified by feline coronavirus (FIP)type I anti-viral antiserum. A microcantilever modified by FIP type I anti-viralantiserum was developed for the detection of FIP type I virus. When the FIP typeI virus positive sample is injected into the fluid cell where themicrocantilever is held, the microcantilever bends upon the recognition of theFIP type I virus by the antiserum on the surface of the microcantilever. Anegative control sample that does not contain FIP type I virus did not cause anybending of the microcantilever. The detection limit of the sensor was 0.1µg ml when the assay time was <1 h.