SARS-CoV-2 Does Not Replicate in Aedes Mosquito Cells nor Present in Field-Caught Mosquitoes from Wuhan

摘要

Replication of SARS-CoV-2 in different cell lines and field mosquitoes collected. SARS-CoV-2 RNA copies detected in Vero E6 cells, C6/36 cells, Aag2 cells, and Sf9 cells evaluated using qRT-PCR at different time points after innoculation. Each cell line was repeated three times. Indirect immunofluorescence assay (IFA) to detect the NP protein of SARS-CoV-2 in the four tested cell lines at 36 h post-inoculation. Bars, 100 µm. Location of mosquito trap sites in the region of Wuhan City, China. Black dots stand for the 13 administrative districts of Wuhan, and Red triangles are the mosquito collection sites.