Rapid Genotypic Detection of rpoB and katG Gene Mutations in Mycobacterium tuberculosis Clinical Isolates from Northern India as Determined by MAS‐PCR

摘要

There is a growing need to develop rapid laboratory research methods to counter the menace of drug resistant tuberculosis (MDR‐TB) cases worldwide especially in developing countries. The present study was undertaken to investigate the type and frequency of and mutations in rifampicin (RIF) and isoniazid (INH) resistant strains respectively of (MTB) circulating in Northern India and to explore the utility of multiplex‐allele‐specific (MAS)‐PCR assay for detection of drug‐resistant MTB isolates in low resource set up. J. Clin. Lab. Anal. 27:31–37, 2013. © 2012 Wiley Periodicals, Inc.