AMPK activation induces mitophagy and promotes mitochondrial fission while activating TBK1 in a PINK1-Parkin independent manner

摘要

The authors gratefully acknowledge Dr Ian Ganley (MRC protein phosphorylation and ubiquitylation unit, University of Dundee) for providing the mito-QC mitophagy reporter construct; Professor Richard Youle (National Institute of Health, Bethesda, USA) for providing the PINK1 knockout cell line; Professor Miratul Muqit for providing the PINK1 antibody; MRC PPU Reagents and Services for providing HALO-UBA TUBE construct for bacterial expression. The authors also acknowledge use of the Mitochondrial Profiling Centre, a core resource supported by the University of Birmingham. This study was supported by the BBSRC funded Midlands Integrative Biosciences Doctoral Training Partnership (MIBTP) and MRC Versus Arthritis Centre for Musculoskeletal Ageing Research. G.G.L was supported by a Wellcome Trust Senior Fellowship (104612/Z/14/Z). D.G.H was supported by an Investigator Award from the Wellcome Trust (204766/Z/16/Z). D.J.H. was supported by MRC (MR/N00275X/1 and MR/S025618/1) and Diabetes UK (17/0005681) Project Grants. This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (Starting Grant 715884 to D.J.H.). The authors declare no conflicts of interest.