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Chemically induced herbicide tolerance in rice by the safener metcamifen is associated with a phased stress response

机译:更安全的甲草胺对水稻化学诱导的除草剂耐受性与阶段性胁迫反应有关

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摘要

The closely related sulphonamide safeners, metcamifen and cyprosulfamide, were tested for their ability to protect rice from clodinafop-propargyl, a herbicide normally used in wheat. While demonstrating that both compounds were equally bioavailable , only metcamifen prevented clodinafop from damaging seedlings, and this was associated with the enhanced detoxification of the herbicide. Transcriptome studies in rice cultures demonstrated that whereas cyprosulfamide had a negligible effect on gene expression over a 4 h exposure, metcamifen perturbed the abundance of 590 transcripts. Changes in gene expression with metcamifen could be divided into three phases, corresponding to inductions occurring over 30 min, 1.5 h and 4 h. The first phase of gene induction was dominated by transcription factors and proteins of unknown function, the second by genes involved in herbicide detoxification, while the third was linked to cellular homeostasis. Analysis of the inducible genes suggested that safening elicited similar gene families to those associated with specific biotic and abiotic stresses, notably those elicited by abscisic acid, salicylic acid, and methyl jasmonate. Subsequent experiments with safener biomarker genes induced in phase 1 and 2 in rice cell cultures provided further evidence of similarities in signalling processes elicited by metcamifen and salicylic acid.
机译:测试了密切相关的磺酰胺安全剂,甲氰菊酯和环丙磺酰胺,它们具有保护大米免受氯丁那普-炔丙基(通常用于小麦的除草剂)保护的能力。尽管证明这两种化合物具有相同的生物利用度,但只有甲卡米芬可以阻止氯丁那普损害幼苗,这与除草剂的解毒作用增强有关。在水稻培养物中进行的转录组研究表明,尽管环丙磺酰胺在暴露4小时后对基因表达的影响可忽略不计,但甲卡米芬却干扰了590个转录本的丰度。甲氧卡米芬的基因表达变化可分为三个阶段,分别对应于30分钟,1.5小时和4小时的诱导。基因诱导的第一阶段由转录因子和功能未知的蛋白质控制,第二阶段由与除草剂解毒相关的基因主导,而第三阶段则与细胞体内平衡有关。对可诱导基因的分析表明,安全性引发了与特定生物和非生物胁迫相关的基因家族,特别是脱落酸,水杨酸和茉莉酸甲酯引起的基因家族。随后在水稻细胞培养中在第1和第2阶段诱导的更安全的生物标记基因的实验提供了进一步的证据,证明甲霜芬和水杨酸引发的信号传导过程相似。

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