Extracellular vesicles derived from human ES-MSCs protect retinal ganglion cells and preserve retinal function in a rodent model of optic nerve injury

摘要

Isolation and characterization of embryonic stem cell-derived mesenchymal stem cells (ES-MSC) extracellular vesicles (EVs). Phase contrast micrograph of passage-9 human embryonic stem cell-derived mesenchymal stem cells (ES-MSCs). Extracellular vesicles (EVs) were isolated from conditioned medium of human ES-MSCs using differential ultracentrifugation. The supernatants (Sup.1–3) were submitted to further centrifugation and the pellets (Pel. 1–3) and Sup. 4 were discarded. Scanning electron microscopy (SEM) of the EVs. Size distribution of ES-MSC EVs was assessed using dynamic light scattering (DLS). Size quantification data demonstrated an average size of 130 nm for the EVs. The Coomassie stained gel of loaded EVs. Representative Western blot analysis for expression of enriched proteins in EVs (two EV membrane markers [CD63 and CD81], and one laminal marker [TSG101])