Scaffold vascularization method using an adipose-derived stem cell (ASC)-seeded scaffold prefabricated with a flow-through pedicle

摘要

Concept of the flow-through prefabrication method ( , ) and study design ( , ): Vascular pedicle types: arteriovenous loop, arteriovenous bundle and flow-through pedicle. New prefabrication method examined by the 3D reconstruction of μCT imaging showing the incision containing the scaffold from one side and its opening and the insertion of the flow-through type vascular pedicle into it. Three groups of six rats each were used: a non-seeded group, an ASC-seeded group and an ASC-seeded osteogenic induced group. Two methods of prefabrication were tested in each rat: classic prefabrication with the vascular pedicle outside the scaffold (vessel ingrowth only from outside) and flow-through prefabrication with the vascular pedicle inside the scaffold with a flow-through pedicle model (vessel ingrowth from outside and inside)