YAP as a key regulator of adipo-osteogenic differentiation in human MSCs

摘要

Effects of DH and LPA on the expression of YAP. Western blot analysis showed the phosphorylated form of YAP in MSCs after treatment with 20 μM DH or 10 μM LPA ( ). Quantification of p-YAP protein levels confirmed a significant increase in p-YAP expression in DH and decreased p-YAP expression in LPA-treated cells ( ). An increase in YAP upon treatment with LPA ( ) and quantification of YAP protein levels confirmed a significant increase in YAP upon LPA treatment ( ). Band intensity was normalized to that of actin (mean ± SEM;  = 3, *  P P t test)