Targeted lung denervation in sheep: durability of denervation and long-term histologic effects on bronchial wall and peribronchial structures

摘要

Representative histochemical section from outside of the TLD treatment zone in a sheep 30 days post-procedure. Grouping of bronchial structures for semi-quantitative analysis. The tissue that makes up and immediately surrounds the airway was divided into four separate structures for analysis. The bronchial epithelium was scored as a single tissue. The bronchial lamina propria, smooth muscle, submucosa, and adventitial layer between airway and alveolar tissue made of the tissue scored as the bronchial wall. The bronchial cartilage was scored independent of the bronchial wall. Bronchial nerves were scored using immunohistochemical analysis and a nerve located in the adventitia that immediately surrounds the airway is shown (scale bar is 0.5 mm)