Crystal structures of N-terminal WRKY transcription factors and DNA complexes

摘要

. (A) Sequence alignments of DBDs of both the N-terminus and C-terminus of eight WRKY proteins from group I based on the crystal structure of AtWRKY1-C (PDB code: 2AYD) (Duan et al., ) and AtWRKY1-N complexed with W-box DNA. Completely conserved residues are highlighted with red boxes, whereas less conserved residues are painted with different colors. (B–D) Overall structures of the N-terminal WRKY proteins (AtWRKY1-N, AtWRKY2-N and AtWRKY33-N) in complex with W-box DNA are shown in two orientations. The direct contacting residues with DNA are shown as sticks. (E) Structural comparison of three N-terminal WRKY proteins and other four WRKY domain structures. The loops between β4 and β5 strands with different conformations are circled by the gray background. (F and G) Distances of β3 and β4 of AtWRKY1-N are compared with those of AtWRKY1-C and AtWRKY4-C respectively. Their distances (Å) are shown next to